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C7 [pC7]
C7 [pC7]
規(guī)格:
貨期:
編號(hào):B193094
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) C7 [pC7]
商品貨號(hào) B193094
Designations C7 [pC7]
Species Homo sapiens, human
Depositors J Mandel
Vector
Construct size (kb): 7.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 2.799999952316284
Tissue: fibroblast GM1415 (48,XXXX) cell line
Gene product: DNA Segment, single copy [DXS28]
Alleles: A1, A2
Insert Size (kb) 2.800
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.0; EcoRI--4.4, 2.8; HindIII--4.6, 1.5, 0.78; PstI--7.0; HincII--3.2, 1.9, 1.2, 0.7.
This probe is located 5-15 cM from the DMD locus. It is informative in the prenatal diagnosis of DMD.
The depositor recommends an agarose gel at 0.9% or less to resolve the allelic fragments and washing conditions of 65C, 0.5X SSC, 0.1% SDS.
The faint band at 3-4 kb is of autosomal origin.
References

Oberle I, et al. Characterization of a set of X-linked sequences and of a panel of somatic cell hybrids useful for the regional mapping of the human X chromosome. Hum. Genet. 72: 43-49, 1986. PubMed: 3002952

Pillers DA, et al. Deletion mapping of Aland Island eye disease to Xp21 between DXS67 (B24) and Duchenne muscular dystrophy. Am. J. Hum. Genet. 47: 795-801, 1990. PubMed: 2220819

Schwartz CE, et al. Allan-Herndon syndrome. II. Linkage to DNA markers in Xq21. Am. J. Hum. Genet. 47: 454-458, 1990. PubMed: 2393020

Thakker RV, et al. Linkage analysis of two cloned DNA sequences, DXS197 and DXS207, in hypophosphatemic rickets families. Genomics 8: 189-193, 1990. PubMed: 1979046

Jean Louis Mandel, personal communication

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