Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
human male forearm skin lesion, Ft Sherman, Panama Canal Zone, 1974
Product Format
frozen
Storage Conditions
Frozen: -70°C or colder for 1 week, vapor phase of liquid nitrogen for long-term storage
Axenic/Xenic
Axenic
Type Strain
no
Comments
Promastigotes.
Karyotype analysis
Medium
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 947: L diphasic blood agar (ATCC Medium 1011) with Locke's solution overlay reduced from 3.0 ml to 1.0 ml
ATCC® Medium 1012: Diphasic blood agar medium
ATCC® Medium 807: Brain heart infusion blood agar
ATCC® Medium 431: Trypanosome medium
Growth Conditions
Temperature: 25°C
Cryopreservation
Harvest cells from a culture which is at or near peak density by centrifugation at 1,300 g for 5 min.
Adjust concentration of cells to 2 x 107/ml in fresh medium.
While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium (broth). The DMSO solution when first prepared will warm up due to chemical heat. The solution should be allowed to return to room temperature prior to use.
Mix the cell preparation and the DMSO solution in equal portions. The final concentration will be 107 cells/ml and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should be no more than 15 min.
Dispense in 0.5 ml aliquots into 1.0 - 2.0 ml sterile plastic screw-capped cryules (special plastic vials for cryopreservation).
Place the ampules in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.)
Store in either the vapor or liquid phase of a nitrogen refrigerator.
To thaw a frozen ampule, place it in a 35°C water bath such that the lip of the ampule remains above the water line. Thawing time is approximately 2 to 3 minutes. Do not agitate the ampule. Do not leave ampule in water bath after thawed.
Immediately after thawing, do not leave in the water bath, aseptically transfer the contents of the ampule into a fresh tube of ATCC medium 1011.
Incubate vertically at 25°C with the cap screwed on tightly.
Maintain as described above.
Name of Depositor
L Hendricks
Chain of Custody
ATCC <-- L Hendricks <-- E. Murray <-- L. Hendricks
Year of Origin
1974
References
Giannini SH, et al. Karyotype analysis of Leishmania species and its use in classification and clinical diagnosis. Science 232: 762-765, 1986. PubMed: 3961502
