Restriction digests of the clone give the following sizes (kb): BamHI--2.9, 2.0; EcoRI--2.9, 2.1; KpnI--2.9, 2.0; MluI--3.0, 2.0; SstI--3.0, 2.0. Plasmid useful for vector construction and generation of marked deletions and insertions in cloned DNA. Plasmid useful for vector construction. Contains a nptII (kanR) cassette flanked by omega elements and multiple cloning sites duplicated in inverted orientation. SphI and PstI sites in the nptII gene were removed by site-directed mutagenesis. |
