Paramyxoviridae, Paramyxovirinae, Rubulavirus, Human parainfluenza virus 4
Deposited As
Parainfluenza 4b
Agent
Human parainfluenza virus 4b
Strain
CH 19503
Applications
Respiratory research
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Deposited as Parainfluenza 4b
Effect on Host
CPE, cell degeneration, clumping and sloughing
Recommended Host
NCI-H292 (ATCC®CRL-1848™)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: 6-11 days
Name of Depositor
JC Hierholzer, Centers for Disease Control and Prevention
Source
Child with respiratory tract infection, Washington, DC, 1962
References
Canchola J, et al. Antigenic variation among newly isolated strains of Parainfluenza type 4 virus. Am. J. Hyg. 79: 357-364, 1964. PubMed: 14159955
Castells E, et al. NCI-H292 as an alternative cell line for the isolation and propagation of the human paramyxoviruses. Arch. Virol. 115: 277-288, 1990. PubMed: 22609024
