| 產品名稱 |
Human echovirus 4 |
| 商品貨號 |
B193748 |
| Classification |
Picornaviridae, Enterovirus, Human enterovirus B |
| Agent |
Human echovirus 4 |
| Strain |
Pesascek |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
This item is a mycoplasma free preparation derived from ATCC VR-34 following treatment to remove mycoplasma. VR-34 will no longer be distributed. |
| Product Format |
frozen |
| Storage Conditions |
?Store at -70°C or colder |
| Comments |
This item is a mycoplasma free preparation derived from ATCC VR-34 following treatment to remove mycoplasma. VR-34 will no longer be distributed. |
| Effect on Host |
Cytopathic effects in tissue culture CPE in the form of highly refractile, rounded cells throughout monolayer or in loose clusters. |
| Growth Medium |
ATCC® Medium 30-2003: Eagle's Minimum Essential Medium (EMEM)
Fetal Bovine Serum
|
| Recommended Host |
Production Host: LLC-Mk2 derivative cells (ATCC CCL-7.1). Recommended Host: ?LLC-Mk2 derivative cells (ATCC? CCL-7.1?). Alternate Hosts: MkK, Mk (testis) and HED cells |
| Growth Conditions |
Plant cells 24 hours in advance and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1 hour at 37.0°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. |
| Name of Depositor |
J.L. Melnick |
| Source |
Fecal specimen from case of aseptic meningitis, Connecticut, 1951. |
| References |
Melnick J. Application of tissue culture methods to epidemiological studies of poliomyelitis. Am. J. Public Health 44: 571-580, 1954. PubMed: 13148397
|
