| Vector Information |
Size (kb): 6.6999998092651370 Vector: YIp33 (plasmid) Construction: pBR322, pYe(Leu2)10 Marker(s):LEU2,ampR Construct size (kb): 6.699999809265137 Features: marker(s): ampR, LEU2
replicon: pMB1 |
| Comments |
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. This was constructed by inserting a 2.2 kb SalI/XhoI fragment containing LEU2 from pYe(Leu2)10 into the SalI site of pBR322. The 5' - 3' orientation of LEU2 is counterclockwise. LEU2, originating from strain X2180-1A, includes a region of Ty1-17. |
| References |
Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030
Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863
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