| 產品名稱 |
pT7SC |
| 商品貨號 |
B194639 |
| Designations |
pT7SC |
| Depositors |
J Campbell, California Institute of Technology |
| Biosafety Level |
1 |
| Host |
Escherichia coli XL1-Blue |
| Vector Information |
Size (kb): 4.12
Vector: pT7SC (plasmid)
Promoters: Promoter T7 (phi10)
Construction: pT7-7
Marker(s): ampR
Replicon: pMB1 |
| Applications |
Expression vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.1; EcoRI/BglI--2.9, 1.2; AvaI--4.1; EcoRI/PvuI--2.6, 1.5; HindIII--4.1. Expression vector permitting complete repression of transcription before induction. Useful for cloning products that may be toxic to the host. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37°C |
| References |
Brown WC, et al. Purification and characterization in the Saccharomyces cerevisiae DNA polymerase delta overproduced in Escherichia coli. J. Biol. Chem. 268: 982-990, 1993. PubMed: 8380419
Brown WC, Campbell JL. A new cloning vector and expression strategy for genes encoding proteins toxic to Escherichia coli. Gene 127: 99-103, 1993. PubMed: 8486292
|
| Shipped |
freeze-dried |
| Shipping Information |
Freeze-dried E. coli containing the plasmid |
