At low stringency (2X SSC, 50% formamide, 37C), silver grains are observed over centromeric regions of a number of chromosomes.
Vector
Construct size (kb): 4.800
Insert
DNA: genomic
Insert lengths(kb): 2.100
Tissue: lymphoblast GM1416 (48,XXXX) cell line
Gene product: DNA Segment, repetitive [D22Z2]
Insert Size (kb)
2.100
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Plasmid in Escherichia coli
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.1; HindIII--3.5, 1.3; BamHI--4.8; PstI--4.8; SalI--4.8.
A 0.73 kb EcoRI/HindIII fragment from the insert has been sequenced partially, demonstrating a repeat of about 171 bp with homology to alpha satellite sequence families.
At low stringency (2X SSC, 50% formamide, 37C), silver grains are observed over centromeric regions of a number of chromosomes. At high stringency (0.2X SSC, 50% formamide, 37C), the signal is predominately at the 22 centromeric region.
References
McDermid HE, et al. Isolation and characterization of an alpha-satellite repeated sequence from human chromosome 22. Chromosoma 94: 228-234, 1986. PubMed: 3769652
