| Vector |
Construct size (kb): 5.900000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pSV7d
Intact vector size: 2.400
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
replicon: pMB1, SV40 ori
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: FGFR1
Other gene symbols: FLT2
Genomic copy number: unique
Gene name: fibroblast growth factor receptor 1 (fms-related tyrosine
kinase 2)
Contains complete coding sequence?: Y
Chromosome: 8; Localization: 8 p12
Tissue: teratocarcinoma cell line Tera-2 clone 13
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.343
Cross references: DNA Seq. Acc.: M34641 Insert lengths(kb): 3.342999935150147 Tissue: teratocarcinoma cell line Tera-2 clone 13 Gene product: fibroblast growth factor receptor 1(fibroblast growth factor receptor 1 (fms-related tyrosine kinase 2)) [FLT2] Target Gene: fibroblast growth factor receptor 1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2, 0.65; KpnI--4.0, 1.85; NcoI/XbaI--3.4, 2.35; PstI--2.4, 1.85, 1.6; SstI--5.9. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.32, 1.34, 1.73, 2.76; BglII--0.93, 2.08, 2.69; EcoRI--0.59; KpnI--1.21, 3.01; PstI--0.16, 1.72; PvuII--0.22, 0.73, 2.25, 2.51; SacI--2.35. Contains the complete coding sequence (nt 10-2472). A 450 bp fragment from the 5' end (encoding the extracellular domain) detects transcripts of 4.0 kb and 4.8 kb. The 3' EcoRI site was destroyed during cloning. |
