Insertion at the HindIII site makes the plasmid unstable. Insertion at the BglII (1.5 kb) or BamHI sites destroys plasmid self-transmissibility.
Insertion at the BclI (0.3 kb), BclI (4.5 kb), BglII (6.1 kb), or BglII (7.2 kb) does not affect plasmid self-transmissibility.
Has demonstrated ability to be maintained in S. Venezuelae, S. Lividans, and S. Avermitilis.
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Shipping Information
Distributed: freeze-dried
Preceptrol®
no
Type Strain
no
Comments
With the HindIII site at 0/11 kb; the plasmid has the following other unique sites: PvuI--0.5, BamH--2.5; EcoRI--5.05; EcoRV--7.5.
Reported to contain plasmid pVE1
Medium
ATCC® Medium 196: Yeast malt extract agar
Growth Conditions
Temperature: 26.0°C
Name of Depositor
Eli Lilly & Co.
Special Collection
ATCC
References
MacNeil T, Gibbons PH. rDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes. US Patent 4,703,009 dated Oct 27 1987
MacNeil T, Gibbons PH. Characterization of the Streptomyces plasmid pVE1. Plasmid 16: 182-194, 1986. PubMed: 3027726
