| 產品名稱 | Influenza B virus |
|---|---|
| 商品貨號 | B196273 |
| Classification | Orthomyxoviridae, Influenzavirus B |
| Agent | Influenza B virus |
| Strain | B/Virginia/ATCC5/2012 |
| Biosafety Level | 2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format | frozen |
| Storage Conditions | -70°C or colder |
| Images |  |
| Comments | This item was isolated in cell culture by three passages in MDCK cells. |
| Effect on Host | CPE, rounding and sloughing |
| Recommended Host | MDCK cells (ATCC® CCL-34™)
|
| Growth Conditions | Temperature: 33°C Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium. Incubation: 3-4 days at 33°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Source | Clinical specimen from a patient in Virginia, 2012. |
| References | WHO Global Influenza Surveillance Network. Manual for the laboratory diagnosis and virological surveillance of influenza. World Health Organization, 2001. IBSN: 9789241548090 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
