Features: insert detection: lacZ' marker(s): ampR replicon: pMB1, f1
Applications
vector permitting production of single-stranded DNA
Comments
Streak to xgal + amp plate and pick a blue colony.
Phagemid with polylinker in lacZ' for production of ssDNA.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Marsh JL, et al. The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32: 481-485, 1984. PubMed: 6099327
