| Vector |
Construct size (kb): 50.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaL47.1
Intact vector size: 42.000
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BamHI, EcoRI
Polylinker sites:
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: lambda
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: FTHL16
Gene name: ferritin, heavy polypeptide-like 16
Contains complete coding sequence?: U
Chromosome: 11; Localization: 11 q13
Tissue: lymphocyte
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 18.0
Cross references: DNA Seq. Acc.: X03486 Insert lengths(kb): 18.0 Tissue: lymphocyte Gene product: ferritin, heavy polypeptide-like 16 [FTHL16] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--20.0, 18.0, 12.0; BamHI--several > 20.0; BglII--20, 7.5 (doublet), 4.0 (doublet), 2.0, 0.35; HindIII--25.0, 18.0, 2.3, 1.8, 1.0; SmaI--25.0, 12.0, 6.6 (doublet), 1.7. The insert represents an intact EcoRI genomic fragment, containing the sequence reported in GenBank accession X03486, with the exception that the insert lacks the G at nt 37 of the sequence record. The gene lies in a human genomic 2.3 kb HindIII fragment or a 4.0 kb BglII fragment. |
| References |
Papadopoulos P, et al. A second human ferritin H locus on chromosome 11. Cytogenet. Cell Genet. 61: 107-108, 1992. PubMed: 1395714
Costanzo F, et al. Structure of gene and pseudogenes of human apoferritin H. Nucleic Acids Res. 14: 721-736, 1986. PubMed: 3003694
|
