ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 1.4; HindIII--3.9; PstI--3.4, 0.5, 0.25.
The insert contains the following restriction sites (kb from the 5' EcoRI site): KpnI--0.371; PstI--0.426, 0.601, 0.807, 1.301; HindIII--1.449.
The complete coding sequence is contained in the sequenced 1.4 kb EcoRI/HindIII fragment.
References
Lin FK. DNA sequences encoding erythropoietin. US Patent 4,703,008 dated Oct 27 1987
Lin FK, et al. Monkey erythropoietin gene: cloning, expression and comparison with the human erythropoietin gene. Gene 44: 201-209, 1986. PubMed: 2877922
Beru N, Payton MN. BglII polymorphism at the human erythropoietin gene. Nucleic Acids Res. 19: 1717, 1991. PubMed: 1674133
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
