| 產品名稱 |
pKLMwoIRM3-1 |
| 商品貨號 |
B196877 |
| Designations |
pKLMwoIRM3-1 |
| Species |
Methanothermobacter wolfei (Winter et al.) Wasserfallen et al. |
| Depositors |
New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc. |
| Vector |
Construct size (kb): 16.4 |
| Insert |
DNA: genomic Insert lengths(kb): 12.0 Gene product: restriction endonuclease MwoI [mwoIM] |
| Insert Size (kb) |
12.0 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Shipped: ?Purified plasmid DNA, 200 ng dried. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--8.8, 4.5, 3.1; EcoRI--5.8, 4.4, 3.6, 1.2, 0.5; HindIII--11.0, 4.6, 0.95. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--9.0; HindIII--10.5, 11.45; NsiI--6.6; SphI--4.9. |
| References |
Lunnen KD, Wilson GG. Method for producing the MwoI restriction endonuclease and methylase. US Patent 5,053,330 dated Oct 1 1991
Lunnen KD, et al. Characterization and cloning of MwoI(GCN7GC), a new type-II restriction-modification system from Methanobacterium wolfei. Gene 77: 11-19, 1989. PubMed: 2663652
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
