| Vector Information |
Size (kb): 6.514 DESCRIPTION OF VECTOR:
Intact vector size: 6.514
Type of vector: plasmid
Cloning sites: XhoI SalI ClaI EcoRI PstI SmaI BamHI SpeI
Polylinker sites: XhoI SalI ClaI HindIII EcoRV EcoRI PstI SmaI BamHI SpeI XbaI
Other unique sites: SacI
Construction: pRS414
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...XbaI, ->, 2155-2219
promoter for expression: ADH, <-, 2219-3719
marker(s): ampR, <-, 4966-5896
replicon: CEN6/ARSH4, ->, 6001-6514 Vector: p414 ADH (plasmid) Promoters: Promoter for expression ADH Construction: pRS414 Marker(s):TRP1,ampR Construct size (kb): 6.514 Features: marker(s): TRP1
marker(s): ampR
promoter for expression: ADH
replicon: CEN6/ARSH4
MCS: XhoI...XbaI
terminator: CYC1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; EcoRI--6.6; XhoI--6.6; SacI/XbaI--3.2, 1.8, 1.5. The wild type ADH promoter is active when cells are grown in glucose media but can be repressed 2-10 fold on non-fermentable carbon sources. Low copy number shuttle expression vector. |
