Restriction digests of the clone give the following sizes (kb): SalI--8.6, 2.2; HindIII--7.7, 3.1; PstI--10.8. The SphI/HindIII fragment corresponding to the rough gene contains the following restriction sites (kb from the 5' end): BamHI--3.02; BglII--5.39; EcoRI--0.91, 4.91; SacI--0.57, 4.19, 5.86; SalI--1.80, 3.91; SmaI--4.43. Vector containing a non-autonomous P-element and incorporating the rough gene (ro) as a dominant marker for P-element mediated gene transfer into the germ line of Drosophila melanogaster. The order of the major features in this plasmid is: HindIII - P-element/Carnegie 4/P-element - XhoI/MCS/SphI - ro gene. |
