| 產品名稱 |
pMK174 |
| 商品貨號 |
B197094 |
| Designations |
pMK174 |
| Species |
Mus musculus, mouse |
| Depositors |
RW Elliott |
| Vector |
Construct size (kb): 5.340000152587891 |
| Insert |
DNA: cDNA Insert lengths(kb): 0.9769999980926514 Tissue: kidney Gene product: DNA segment, Chr 17, Roswell Park-17 [RP1] Alleles: b, d, b, d, c, h, g, m, c, h, g, m |
| Insert Size (kb) |
0.977 |
| Media |
ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
A BglI/ScaI double digest removes the insert in 2 fragments, one of about 530 bp containing 120 bp from pBR322 (BglI/ScaI), and one of about 750 bp containing 238 nucleotides from pBR322 (ScaI/ScaI). The insert contains the following sites (in bp from the PstI site): HincII--300; PstI--340, 600; ScaI--430; RsaI--405, 430, 500, 900; AvaI--830. Restriction digests of the clone give the following sizes (kb): AvaI--3.2, 2.5; EcoRI--5.4; PstI--5.0, 0.38 + smaller. D17Rp17 maps to proximal 17. The site on chomosome 4 is on mid 4. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Mann EA, et al. Genetic analysis of a mouse T complex locus that is homologous to a kidney cDNA clone. Genetics 114: 993-1006, 1986. PubMed: 3792827
Rosemary W Elliott, personal communication
|
