Restriction digests of the clone give the following sizes (kb): BamHI--8.8, 0.6; EcoRI--6.5, 2.3, 0.86; HindIII--7.6, 1.9; PstI--5.6, 2.1, 1.3, 0.6; XhoI--9.4. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--1.25, 1.77; BglI--0.74; EcoRV--0.86; KpnI--0.54; PstI--0.04, 0.57; SmaI--0.06, 0.21, 1.27, 1.30. Includes approximately 482 bp of 5' untranslated sequence, the complete coding sequence and approximately 210 bp of 3' untranslated sequence. The insert is in the forward orientation relative to the CMV promoter. The order of the major features of the plasmid is: ampR - EcoRI - SV40 ori - SV40 promoter - XhoI - hygR - tk polyadenylation - TaqI - SV40 polyadenylation - EcoRI - CMV promoter - HindIII - insert - HindIII - SalI - bGH polyadenylation - PvuI. |
