Restriction digests of the clone give the following sizes (kb): BamHI--13.0; EcoRI--13.0. Below 37C, there is one copy per genome equivalent stabilized by the parB locus. At 42C replication is uncontrolled because induction of the cI857-regulated PR promoter permits expression of the copB, copA, and repA genes. A promoter-cloning vector using increased expression of beta-galactosidase as the reporter. |
