| 產(chǎn)品名稱 |
Mu Islet (E6/E7) |
| 商品貨號 |
B197684 |
| Organism |
Mus musculus, mouse |
| Tissue |
pancreas/islet cells; HPV16 E6/E7 transformed |
| Product Format |
frozen |
| Morphology |
epithelial-like |
| Culture Properties |
adherent |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
8 - 12 weeks |
| Gender |
male |
| Strain |
C57BLKS/J.j |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
CRL-2999 was established from isolated mouse Islets of Langerhans. The Islet cells were immortalized by transduction with pLXSN-E6E7 (ATCC CRL-2203) and selected with 400 µg/mL G418. |
| Comments |
At ATCC, the cells have been shown to form small clusters when cultured in collagen gel. The cell clusters stain positive with Newport Green which would suggest some level of insulin expression. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm 2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping, do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1 X 104 to 2 X 104 viable cells/cm2 is recommended.
- Incubate cultures at 37°C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:4 is recommended.
Medium renewal: every 2 to 3 days
|
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
ATCC |
| Year of Origin |
March 2009 |
