| Comments |
The insert contains the following sites separated by (kb) (approximately): SacI- 0.3- KpnI- 1.3- KpnI- 1.0- BamHI- 1.2- XbaI- 0.8- BamHI- 0.4- PstI- 0.4- XbaI- 0.4- BamHI- 0.4- PstI- 0.4- KpnI- 0.5- SacI. Restriction digests of the clone give the following sizes (kb): SalI--27.7; EcoRV--12.8, 4.4, 3.3 (doublet), 1.5, 1.2, 1.0; XbaI--17.0, 5.6, 3.3, 1.7; BamHI--23.1, 2.2, 1.7; SmaI/SalI--13.1, 8.6, 3.4, 0.6. MSM1 is the nuclear gene encoding the mitochondrial methionyl-tRNA synthetase. The smallest region capable of complementing a msm1-1 mutation is defined by a KpnI-PstI fragment of approximately 3 kb from the middle of the insert, and spanning the BamHI and XbaI sites separated by 1.2 kb. The probe, pG72/T1, has been deposited in Escherichia coli RR1 as ATCC 37663 and in Saccharomyces cerevisiae C122/U3 as ATCC 66319. |
| References |
Tzagoloff A, et al. Characterization of MSM1, the structural gene for yeast mitochondrial methionyl-tRNA synthetase. Eur. J. Biochem. 179: 365-371, 1989. PubMed: 2645139
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