This Schizosaccharomyces pombe expression vector is useful for constructing fusion proteins with a C-terminal GFP tag. Promoter is nmt. GFP sequence corresponds to Genbank Accession number U87624 with nucleotides 32-747 of GFP matching nucleotides 21-736 of U87624, with additional modification S65T by changing codon to ACC. The order of the major feature in the plasmid is: nmt promoter - XhoI - BglII - SacII - NotI - TTG - GFP - TAG - SalI - SmaI - termination sequence - arsI - pUC119 (3.3 kb) - ura4. Restriction digests of the vector gave the following bands (in kb): EcoRI - 8.1, 1.2; SacI - 9.3; XhoI/SmaI - 8.5, 0.75. -- ATCC staff |
