| 產品名稱 |
pWE16 |
| 商品貨號 |
B197851 |
| Designations |
pWE16 |
| Depositors |
GA Evans |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 8.8000001907348630 Vector: pWE16 (cosmid) Promoters: Promoter T3 Construction: pCV107, pCV108, pJB8 Marker(s):ampR,mtxR Construct size (kb): 8.800000190734863 Features: marker(s): ampR, mtxR
promoter: T3, T7
replicon: pMB1 |
| Applications |
contains easily purifiable cassette(s) for construction expression vector integrating vector shuttle vector vector for constructing genomic libraries vector permitting RNA synthesis in vitro vector permitting positive selection for integration |
| Comments |
pWE15 (ATCC 37503) and pWE16 (ATCC 37524) differ only in the markers selectable in vertebrate cells. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Useful for constructing genomic libraries and chromosome walking and for gene transfer using dihydrofolate reductase as a selectable marker. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 30.0°C |
| References |
Wahl GM, et al. Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. Proc. Natl. Acad. Sci. USA 84: 2160-2164, 1987. PubMed: 3031668
Evans GA, Wahl GM. Cosmid vectors for genomic walking and rapid restriction mapping. Methods Enzymol. 152: 604-610, 1987. PubMed: 2443810
|
| Shipped |
freeze-dried |
| Shipping Information |
Distributed: freeze-dried |
