| Vector |
Construct size (kb): 4.5 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene name: cell surface glycoprotein
Contains complete coding sequence?: N
Chromosome: X; Localization: X, 39.2800000 cR
Genome: Homo sapiens
Gene symbol: DXS1692E
Gene name: DNA Segment, single copy, expressed
Contains complete coding sequence?: N
Chromosome: X; Localization: X; X, 39.2800000 cR
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.2
Cross references: DNA Seq. Acc.: D29808; EST: EST06986
Nucleotides 1-336 of the insert correspond to
nucleotides 584-919 of D29808 with 99% identity.
DNA Seq. Acc.: D29808; EST: EST06985
Nucleotides 262-1 of the insert correspond to
nucleotides 1466-1727 of D29808 with 97% identity.
DNA Seq. Acc.: T09092; EST: EST06985; Primer: M13-21; THC
id: THC123088
DNA Seq. Acc.: T09093; EST: EST06986; Primer: M13 Reverse;
THC id: THC123088 Insert lengths(kb): 1.200000047683716 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: DNA Segment, single copy, expressed [DXS1692E] |
