| 產(chǎn)品名稱 |
pCMV-SCAP |
| 商品貨號(hào) |
B198610 |
| Designations |
pCMV-SCAP |
| GenBank Number |
U67060
|
| Depositors |
JL Goldstein, University of Texas Southwestern Medical Center |
| Applications |
In another host, produces protein SREBP cleavage-activating protein |
| Vector |
Name of construct: pCMV-SCAP
Excise insert: AseI + XbaI
Promoter giving sense strand: CMV
Size of construct (kb): 10.4
Markers: ampR, G418R
Name of vector: pRc/CMV7SB
Intact vector size: 6.2
Type of vector: phagemid
Vector end: SalI
Vector end: XbaI
Cloning sites: SalI notI
Polylinker sites: SalI KpnI SacI BamHI EcoRI PstI EcoRV NotI XhoI AvaI XbaI SacI
Host range: Escherichia coli, mammalian cells
Features (with orientation and position when available):
promoter for expression: CMV, →
other: adenovirus/immunoglobulin hybrid intron, →
MCS: SalI...SacI, →
terminator: BGH polyadenylation, →
replicon: f1
promoter: SV40, →
replicon: SV40
marker(s): neoR, →
terminator: SV40 polyadenylation, →
replicon: f1
marker(s): ampR, → |
| Insert |
Genome: hamster
Gene symbol: SCAP*
Gene name: SREBP cleavage-activating protein
Contains complete coding sequence?: Y
Type of DNA: cDNA
Insert size (kb): 4.2
Cross reference: DNA seq. Acc.: U67060 |
| Insert Size (kb) |
4.20 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): AseI/XbaI--4.5, 2.4, 1.4, 1.2, 0.64, 0.25; BamHI--8.1, 2.3; EcoRI--5.0 (doublet), 0.39. |
| References |
Hua X, et al. Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein. Cell 87: 415-426, 1996. PubMed: 8898195
|
