| 產品名稱 |
pCWA274 |
| 商品貨號 |
B198651 |
| Designations |
pCWA274 |
| GenBank Number |
L27425
|
| Species |
Homo sapiens, human |
| Depositors |
CW Anderson |
| Vector |
Construct size (kb): 10.35000038146973 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript KS+
Intact vector size: 2.964
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: SacII XmaII NotI XbaI SpeI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI (SalI HincII AccI) XhoI DraII ApaI KpnI
Polylinker sites: SEE COMMENTS
Construction: pUC19
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
terminator: none
Cross references: |
| Insert |
DNA: genomic DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: DNA-PK(CS)
Gene name: DNA-activated serine/threonine protein kinase (catalytic
subunit)
Contains complete coding sequence?: N
Chromosome: 8; Localization: 8 q11
Type of DNA: genomic
Insert end: EcoRI
Insert end: EcoRI
Insert size (kb): 7.392
Cross references: DNA Seq. Acc.: L27425
Nucleotides 1-7393 of the insert correspond to
nucleotides 247-7693 of L27425. Insert lengths(kb): 7.392000198364258 Gene product: DNA-activated serine/threonine protein kinase (catalytic subunit) [DNA-PK(CS)] Alleles: -, + |
| Insert Size (kb) |
7.392 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--7.4, 3.0; BamHI--10.4; BglII--9.3, 1.05; HindIII--6.0, 4.4; SmaI--8.0, 2.4. The insert contains the following restriction sites (approximate kb from the 5' end): SacI--3.06, 5.68; BglII--5.15, 6.00; SmaI--5.26; HindIII--6.01. PvuII polymorphism was detected in the PCR product generated with upstream primer (nt 6229-6252 of L27425) and downstream primer (compliment of nt 7570-7589). |
| References |
Sipley JD, et al. Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8. Proc. Natl. Acad. Sci. USA 92: 7515-7519, 1995. PubMed: 7638222
|
