| 產品名稱 |
JC polyomavirus |
| 商品貨號 |
B198689 |
| Classification |
Polyomaviridae, Polyomavirus |
| Deposited As |
JC virus |
| Agent |
JC polyomavirus |
| Strain |
MAD-4 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Derived by adaptation from SVG p12 cells (ATCC® CRL-8621™) product ATCC® VR-1397™ to COS-7 cells (ATCC® CRL-1651™). ATCC® VR-1397™ and ATCC® VR-1583™ have not been compared for sequence or infectivity in tissue cultures. This strain is highly neurooncogenic and differs from MAD-1 strain, in part by a 19 bp deletion that eliminates the TATA box. JC polyomavirus hemagglutinates human type O red blood cells (HA requires addition of 0.25% sodium deoxycholate). |
| Effect on Host |
No CPE, viral proteins detectable by antibody |
| Recommended Host |
COS-7 (ATCC® CRL-1651™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 70-80% confluent. Freeze-thaw viral material 6 times prior to inoculation. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 7 days |
| Name of Depositor |
EO Major, National Institute of Neurological Disorders and Stroke; ATCC? |
| Source |
Brain tumor of owl monkey |
| References |
Frye S, et al. Efficient production of JC virus in SVG cells and the use of purified viral antigens for analysis of specific humoral and cellular immune response.. J. Virol. Methods 63: 81-92, 1997. PubMed: 9015278
Major EO, et al. Owl monkey astrocytoma cells in culture spontaneously produce infectious JC virus which demonstrates altered biological properties. J. Virol. 61: 1435-1441, 1987. PubMed: 3033272
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