ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains the following restriction sites (nucleotide position relative to published sequence): ClaI--250; EcoRI--263; HindIII--914; SacI--472; StuI--612.
Restriction digests of the clone give the following sizes (kb): PstI--4.4, 1.0; EcoRI--3.95, 1.48; HindIII--4.5, 0.9; BglI--2.95, 2.35; SacI--5.4, ClaI--4.0, 1.5; ClaI+HindIII--4.0, 0.9, 0.65; SstI+HindIII--4.2, 0.9, 0.45.
The insert contains the complete cDNA with the coding region extending from 59 to 601. There is a signal peptide of 19 aa. The introns are positioned after the following nucleotides: I--157; II--291; III--365; IV--476; V--578; VI--624.
References
Laperche Y, et al. Tissue-specific control of alpha2u globulin gene expression: constitutive synthesis in the submaxillary gland. Cell 32: 453-460, 1983. PubMed: 6186396
Sarkar P, Feigelson P. Developmentally regulated male-specific transfactor(s) enable in vitro transcription of a cloned alpha 2u-globulin gene. Mol. Endocrinol. 3: 342-348, 1989. PubMed: 2469008
Choy HA, et al. Nuclear factors from expressing tissues interact in vitro with a rat alpha-2u globulin gene intron. J. Biol. Chem. 264: 1754-1759, 1989. PubMed: 2463987
