| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--20.0, 9.6, 5.3, 3.9, 3.5, 2.9, 1.6; HindIII--23.0, 8.8, 6.0, 4.4, 3.8, 0.55; ClaI--12.0, 6.0, 4.0 (doublet), 3.6, 3.3, 2.8, 2.6, 2.3, 2.2, 1.9, 1.1, 0.6; SacI--25.0, 20.0; SmaI--22.0, 10.0, 8.4, 5.8. Requires a supE host for propagation. In hosts lacking a nonsense suppressor, lysogenization and lytic growth of the phage are prevented by the cI- Int- and P- phenotypes of the bacteriophage (partial genotype: cI857 susP80 nin5 b522 plac5). For insertional mutagenesis of bacterial or yeast genes carried in plasmid vectors. The promoterless lacZ sequence contains an EcoRI site (3070 nt from the IS10R end). Constructed from lambdaNK780 and pTn10-LLK. The kanamycin resistance element is from Tn903. Contains the mini-Tn10-LLK insert that consists of the following features: IS10R - promoterless lacZ element starting at codon 8 - LEU2 - kanR - IS10L. |
| References |
Lin Y, et al. Meiotically induced rec7 and rec8 genes from Schizosaccharomyes pombe. Genetics 132: 75-85, 1992. PubMed: 1339382
Riles L, et al. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6-kilobase pairs. Genetics 134: 81-150, 1993. PubMed: 8514151
Reenan RA, Kolodner RD. Isolation and characterizatin of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins. Genetics 132: 963-973, 1992. PubMed: 1459447
Gerald R Smith, personal communication
|
