| Comments |
Reported to contain EcoRI/HindIII fragments of the following sizes (kb): 7.72, 3.00 (vector-associated); 3.92, 3.39, 3.02, 2.28, 2.18, 2.08, 2.08, 1.95, 1.65, 1.52, 1.52, 1.47, 0.86, 0.8, 0.78, 0.76, 0.50, 0.50. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. Corresponds to nt 443830-476199 of the sequence of chromosome VIII. |
| References |
Johnston M, et al. Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. Science 265: 2077-2082, 1994. PubMed: 8091229
Linda Riles, personal communication
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