New England Biolabs, Inc., I Schildkraut, New England Biolabs, Inc.
Applications
produces protein DNA polymerase (Vent)
Vector
Construct size (kb): 10.10
Insert
DNA: genomic
Insert lengths(kb): 3.90
Gene product: DNA polymerase (Vent) [pol]
Target Gene: DNA polymerase (Vent)
Insert Size (kb)
3.900
Media
ATCC® Medium 1949: LB medium (ATCC medium 1065) with 50 mcg/ml chloramphenicol and 100 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
Restriction digests of pNEB671 give the following sizes (kb): BamHI--10.5; BamHI/NdeI--6.2, 2.1 doublet; EcoRI--9.0, 1.9; HindIII--9.0, 0.7, 0.7. pLysS is also detected at 4.9 kb (or 4.2 and 0.6 kb with BamHI).
Constructed by modifying the sequence TTTATG to CATATG to create a NdeI site at the 5' end (nt 288-293), and by removing the intervening sequence spanning nucleotides 1776 and 3398 of the sequence record.
References
Comb DG, et al. Purified thermostable DNA polymerase obtainable from Thermococcus litoralis. US Patent 5,322,785 dated Jun 21 1994
Comb DG, et al. Recombinant thermostable DNA polymerase from archaebacteria. US Patent 5,352,778 dated Oct 4 1994
Perler FB, et al. Intervening sequences in an Archaea DNA polymerase gene. Proc. Natl. Acad. Sci. USA 89: 5577-5581, 1992. PubMed: 1608969
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
