Restriction digests of the clone give the following sizes (kb): HindIII-3.5; BamHI--3.5; EcoRI--3.5; SmaI--3.5. Commercial use of the human cytomegalovirus promoter/enhancer has been patented on behalf of B. Fleckenstein and W. Schaffner by the Beringwerke. Interested parties should contact the patent holders before planning a commercial application. A shuttle vector for transient expression of cDNA inserts in mammalian cells. Constructed by inserting a 0.62 kb SmaI/EcoRI fragment from pMThGH containing part of exon 5 and 3' untranslated regions of the human growth hormone gene into the SmaI/EcoRI sites of pAVE1 (ATCC 37838). |
