Restriction digests of the clone give the following sizes (kb): PvuII--4.3, 0.2; HincII--3.0, 1.5; EcoRI--4.5. A plasmid shuttle vector for investigating promoter and/or enhancer elements. The sequence at the junction of the multiple cloning site and CAT is GAATTAGCTT and at the junction of SV40 and SP6 is GGATCAATTC. The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and pGCAT-C (ATCC 37641). The BamHI/HindIII fragment of pSB1 containing CAT was cloned into the EcoRI site of pGEM-4 (all ends blunt). Contains the SV40 polyadenylation site and donor and acceptor sites of SV40 small t antigen intron. Has a polylinker upstream of the reporter CAT gene and demonstrates low basal CAT expression in eukaryotic cells. |
