The sequence surrounding the polylinker is known so that inserts can be sequenced directly.
pLS1 and its derivatives can replicate in Streptococcus pneumoniae, S. oralis, Bacillus subtilis, and E. coli.
Contains the complete coding sequence for lytA. The 3' HindIII site is approximately 50 bp beyond the termination codon.
The polylinker sites are upstream of the promoter-less lytA gene. There are stop codons in all 3 reading frames between the cloning sites and the initiation codon.
The ScaI and EcoRV sites interrupt the tetracycline resistance gene.
A HindIII fragment containing polylinker sequences from pUC19 and an XbaI/HindIII fragment containing the promoterless gene was cloned into the HindIII site of pLES1. The XbaI site was created 18 bp upstream of the initiation codon. |
