The insert contains the following restriction sites (in bp from the 5' end): AccI--347; AvaI--230; BglI--572; ScaI--256; SmaI--230. Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 1.2; EcoRI--uncut; HindIII--4.0; PstI--2.5, 1.4; SacI--2.4, 1.5. The insert encodes 224 amino acids of H and contains the 3' untranslated region (229 bp). |
