| Applications |
Highly polymorphic locus with RFLPs observed with BclII, BglI, BglII, BstNI, MspI, PstI, PvuII, SacI and TaqI. Most seem to be site mutations. |
| Insert |
DNA: genomic Insert lengths(kb): 18.0 Gene product: DNA Segment, single copy [D16S20] Alleles: A1, B1, D1, B2, A2, D2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): 18.0 + arms. This probe has been assigned to a linkage group on 16q (Lathrop et al., HGM9). The order of marker loci is cen-HP-CTRB-D16S20-pLC9-(APRT,D16S7)-qter. Highly polymorphic locus with RFLPs observed with BclII, BglI, BglII, BstNI, MspI, PstI, PvuII, SacI and TaqI. Most seem to be site mutations. Sites A, B, and D show a heterozygosity of 86% (sample of 36 unrelated individuals). The whole phage works well as a probe after prehybridization with total human DNA to block annealing of repetitive sequences. The final stringency wash is 0.1X SSC/ 0.1% SDS at 65C. |
| References |
Julier C, et al. A primary map of 24 loci on human chromosome 16. Genomics 6: 419-427, 1990. PubMed: 2328986
Maslen C, et al. An unusual clustering of restriction site polymorphisms at D16S20. Cytogenet. Cell Genet. 46: 656, 1987.
Michael Litt, personal communication
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