| Vector Information |
Size (kb): 6.3070001602172850 DESCRIPTION OF VECTOR:
Intact vector size: 6.307
Type of vector: plasmid
Cloning sites: SpeI BamHI SmaI PstI EcoRI ClaI SalI XhoI
Polylinker sites: XbaI SpeI BamHI SmaI PstI EcoRI EcoRV HindIII ClaI SalI XhoI
Construction: pRS424, GAL1 promoter
Host range: Saccharomyces cerevisiae; Escherichia coli
Features (with orientation and position when available):
marker(s): TRP1, ->, 200-1213
terminator: CYC1, ->, 1895-2155
MCS: XhoI...SpeI, ->, 2155-2212
promoter for expression: GAL1, <-, 2219-2679
marker(s): ampR, <-, 3820-4760
replicon: 2 micron, ->, 4860-6200 Vector: p424 GAL1 (plasmid) Promoters: Promoter for expression GAL1 Construction: pRS424, GAL1 promoter Marker(s):TRP1,ampR Construct size (kb): 6.307000160217285 Features: marker(s): TRP1
marker(s): ampR
promoter for expression: GAL1
replicon: 2 micron
MCS: XhoI...SpeI
terminator: CYC1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): SacI/XbaI--3.2, 1.7, 1.0, 0.45; EcoRI--6.3; BamHI--6.3. Expression from the galactokinase (GAL1) promoter is tightly repressed by glucose and is strongly induced by galactose. High copy number shuttle expression vector. One of 32 yeast expression vectors (ATCC 87318-87349) differing in promoter, selection marker and replicon. |
