Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Type Strain
no
Genotype
MATa, his3delta1, leu2delta0, met15delta0, URA3
Preceptrol®
no
Mating Type
a
Comments
Temperature sensitive
HHF1 modfied to H4S47C via site directed mutagenesis. The second histone H4 gene, HHF2, was knocked out and replaced with the URA3 selection marker.
Morphology
On YEPD medium at 25°C after 5 days cells are globose, ovoidal or elongate and are usually isolated or in small groups. Growth is butyrous and light cream colored.
Medium
ATCC® Medium 1069: YPAD medium
ATCC® Medium 1245: YEPD
ATCC® Medium 200: YM agar or YM broth
Growth Conditions
Temperature: 20°C to 25°C
Atmosphere: Typical aerobic
Name of Depositor
K Brogaard, J Widom
Special Collection
ATCC
Chain of Custody
ATCC <-- K Brogaard, J Widom
Isolation
Targeted mutagenesis from BY4741.
References
Brogaard K, et al. A map of nucleosome positions in yeast at base-pair resolution. Nature 486(7404): 496-501, 2012. PubMed: 22722846
