| Vector |
Construct size (kb): 6.400000095367432 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCDM8
Intact vector size: 4.356
Type of vector: phagemid
Vector end: BstXI
Vector end: BstXI
Cloning sites: HindIII XbaI XhoI BstXI PstI NotI
Polylinker sites: HindIII XbaI XhoI BstXI --stuffer--BstXI XhoI PstI NotI XbaI
Construction: CMV, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
replicon: pMB1, 1-587
replicon: M13, <-, 588-1182
marker(s): supF, <-, 1183-1384
promoter: CMV, ->, 1516-2170
promoter for in vitro transcription: T7, ->, 2171-2189
MCS: HindIII...XbaI, ->, 2198-2655
terminator: SV40 splice site and polyadenylation, ->, 2656-3352
replicon: polyoma, 3353-4188
replicon: SV40, 4189-4352
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KHK
Gene name: ketohexokinase (fructokinase)
Contains complete coding sequence?: Y
Tissue: Hep G2 cell line
Disease: hepatocellular carcinoma
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 1.899
Cross references: DNA Seq. Acc.: X78678
Nucleotides 1-1899 of the insert correspond to
nucleotides 1-1899 of X78678. Insert lengths(kb): 1.89900004863739 Tissue: Hep G2 cell line hepatocellular carcinoma Disease: hepatocellular carcinoma Gene product: ketohexokinase (fructokinase) [KHK] |
| Comments |
Restriction digests of the clone give the following sizes (kb): XbaI--4.3, 2.1; HindIII--6.4; BamHI--3.9, 2.4; SacI--4.7, 0.87, 0.7; PstI--4.7, 1.1, 0.5. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.41, SacI--0.54, 1.36; PstI--0.46, 0.88. Derived from the form A variant transcript (alternately spliced first coding exon). The second polyadenylation signal was used. |
