Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
This strain is being made readily available to the scientific community with the proviso that it not be used to form the basis of a product for commercial sale or be included in any commercial product (personal communication).
Constructed from JC7623 by P1 transduction of delta(mcrBC-hsdRMS-mrr) from Escherichia coli ER1648.
Methylation tolerant, recombination deficient host for bacteriophage lambda. Capable of maintaining sequences that form secondary structure
Not suitable as a plasmid host due to the formation of plasmid multimers
Methylation tolerant, recombination deficient host for bacteriophage lambda. Capable of maintaining sequences that form secondary structure.
Not suitable as a plasmid host due to the formation of plasmid multimers.
Constructed from JC7623 by P1 transduction of delta(mcrBC-hsdRMS-mrr) from E. coli ER1648.
This strain is being made readily available to the scientific community with the proviso that it not be used to form the basis of a product for commercial sale or be included in any commercial product.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Name of Depositor
JP Doherty
References
Doherty JP, et al. Escherichia coli host strains SURE (TM) and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains. Gene 124: 29-35, 1993. PubMed: 8440479
