Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Derived from same strain by passaging in the absence of bovine serum. Passage infected cells for optimal propagation of virus.
Effect on Host
No CPE, detect by immunofluorescence or molecular-based procedures
Recommended Host
BT (ATCC® CRL-1390™)
Growth Conditions
Recommendations for Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 60% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 4-21 days at 37°C in a humidified 5% CO2 atmosphere.
Name of Depositor
M Snyder, GA Erickson, The National Animal Disease Center
Special Collection
ATCC
Source
Spleen from calf with diarrhea, New York, 1948
References
Baker JA. . Am. J. Vet. Res. 15: 525, 1954.
Kim SG, Dubovi EJ. A novel one-step single-tube RT-duplex PCR method with an internal control for detection of bovine viral diarrhea virus in bulk milk, blood, and follicular fluid samples. Biologicals. 31: 103-106, 2003. PubMed: 12770539
