| 產品名稱 |
Human adenovirus 37 |
| 商品貨號 |
B202342 |
| Classification |
Adenoviridae, Mastadenovirus, Human adenovirus D |
| Deposited As |
Adenovirus type 37 |
| Agent |
Human adenovirus 37 |
| Strain |
GW (76-19026) |
| Applications |
Respiratory research |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1 mL per vial |
| Storage Conditions |
-70°C or colder |
| Comments |
Good immunogen in rabbits and horses
Cross-reacts with Human adenovirus 10, 13, 19 and 30 by serum neutralization and HAI
HA with rat, rhesus monkey and human O type red blood cells |
| Effect on Host |
CPE, refractile rounding and sloughing |
| Recommended Host |
WI-38 (ATCC® CCL-75™) |
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plate cells 24 hours prior to infection and infect when cultures are 75-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere while rocking continuously. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 2-11 days at 37°C in a humidified 5% CO2 atmosphere, until CPE are well advance through 90% of the culture. |
| Name of Depositor |
JC de Jong, National Institute for Public Health and the Environment, Netherlands |
| Source |
Human eye with keratoconjunctivitis (possible genitourinary disorder), Netherlands, 1976 |
| Year of Origin |
1976 |
| References |
Hierholzer JC, et al. Antigenic relationships among the 47 human adenoviruses determined in reference horse antisera. Arch. Virol. 121: 179-197, 1991. PubMed: 1759904
de Jong JC, et al. Adenovirus 37: identification and characterization of a medically important new adenovirus type of subgroup D. J. Med. Virol. 7: 105-118, 1981. PubMed: 7264611
Ma Y, Mathews MB. Structure, function, and evolution of adenovirus-associated RNA: a phylogenetic approach. J. Virol. 70: 5083-5099, 1996. PubMed: 8764016
Wadell G, et al. Characterization of candidate adenovirus 37 by SDS-polyacrylamide gel electrophoresis of virion polypeptides and DNA restriction site mapping. J. Med. Virol. 7: 119-125, 1981. PubMed: 6267186
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| Cross References |
Nucleotide (GenBank) :
U52553
Human adenovirus type 37 virus-associated RNA pre-terminal protein (pTP) and 52,55K protein genes, partial cds.
Nucleotide (GenBank) :
AF086569
Human adenovirus type 37 early E3B 10.4 kDa protein, early E3B 14.5 kDa protein, and early E3B 14.7 kDa protein genes, complete cds.
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