Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Preceptrol®
no
Genotype
thi-1 endA1 hsdR17 lacIq delta(lacZ)M15 supE44 dam-13::Tn9 F'
Type Strain
no
Comments
Tn10 was lost spontaneously.
Occasionally streak onto minimal medium and reisolate single colonies to assure M13 sensitivity.
Derived from DG98 (MM294) by P1 transduction and chloramphenicol selection; isolated by D. Gelfand.
A host strain for the preparation of dam- M13 DNA.
Medium
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
Name of Depositor
JC Hunter-Cevera
References
Lawyer FC, et al. Isolation, characterization, and expression in Escherichia coli of the DNA polymerase gene from Thermus aquaticus. J. Biol. Chem. 264: 6427-6437, 1989. PubMed: 2649500
Marinus MG, et al. Insertion mutations in the dam gene of Escherichia coli K-12. Mol. Gen. Genet. 192: 288-289, 1983. PubMed: 6316110
