The insert includes the following restriction sites (in nucleotide positions from the 5' end of the published sequence): BamHI--748, 1083, 1177; PstI--1586. Restriction digests of the clone give the following sizes (kb): BamHI--2.8, 0.7, 0.5; EcoRI--2.8, 1.3; HindIII--2.8, 1.3; PstI--3.6, 0.4; XbaI--4.3. The insert extends from nucleotide 558 to 1871 of the published sequence, and encodes the carboxy terminal portion of the protein for which the coding sequences end at 1495. This detects a mRNA of about 5.1 kb in normal and acute-phase rat liver. |
