| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--12.5, 9.6, 9.4, 7.9, 6.6; HindIII--15.5, 10.0, 7.2, 3.5, 3.2, 2.6, 2.3, 1.9; BglII--25, 20; SalI--25, 10.5, 9.1; SfiI--21, 15, 9.0; EcoRI/HindIII--11.5, 6.8 (doublet), 4.0, 3.8, 3.3, 2.8, 2.0, 1.8, 1.2; consistent with deposited DNA. Codominant segregation of the MspI RFLP was observed in 54 three-generation families. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs were observed under total human DNA prehybridization and hybridization. |
| References |
Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence (cMCOE32) on chromosome 2 [D2S53]. Nucleic Acids Res. 16: 6258, 1988. PubMed: 2899880
Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220
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