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Construct size (kb): 3.799999952316284

DNA: genomic

Insert lengths(kb): 0.800000011920929

Tissue: embryonic lung fibroblast WI-38 (serum-induced)

Gene product: cytokine A2, small inducible( monocyte chemotactic protein, monocyte chemotactic protein 1, homologous to mouse Sig-je, small inducible cytokine A2, small inducible cytokine subfamily A (Cys-Cys), member 2, small inducible gene JE) [JE*]

The insert contains the following sites (with nucleotide positions according to the published sequence): NaeI at -426; HindIII at -420; SacI at -408 and PstI at -307.

Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 0.8; HindIII--3.65, 0.15; BamHI--3.8; PstI--3.8; XbaI/SacI--2.9, 0.7, 0.2.

The 5' clone phgJE3-3 (ATCC 61366/61367) and the 3' clone phgJE2-1 (ATCC 61368/61369) together generate a complete gene.

This insert was subcloned from lambdahJE-7 and extends from -531 relative to the mRNA cap site into the first intron which begins as +228. It is oriented in this clone with transcription from the SP6 promoter giving the + strand.