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pHA10
pHA10
規(guī)格:
貨期:
編號:B203388
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pHA10
商品貨號 B203388
Designations pHA10
Depositors A Honigman
Biosafety Level 1
Vector Information
Size (kb): 11.3900003433227500
Vector: pHA10 (plasmid)
Promoters: Promoter none
Construction: pBR322, lambdaEcoRI-D
Marker(s):ampR
Construct size (kb): 11.39000034332275
Features: insert detection: lambdakil
marker(s): ampR
promoter: none
replicon: pMB1
repressor gene: cI857
Applications
vector permitting positive selection for inserts
Comments
Temperature sensitive at 42C for viability.
A positive selection vector for DNA inserts controlled by lambdaPL through the regulation of the kil gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Honigman A, et al. Plasmid vectors for positive selection of DNA inserts controlled by the lambda pL promoter, repressor and antitermination function. Gene 13: 289-298, 1981. PubMed: 6266919

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