| 產(chǎn)品名稱 |
Saccharomyces cerevisiae AB320 genomic library in YEp13 Escherichia coli |
| 商品貨號 |
B203668 |
| Designations |
Saccharomyces cerevisiae AB320 genomic library in YEp13 Escherichia coli |
| GenBank Definition |
Saccharomyces cerevisiae sec11-7 suppressor Spc1p (SPC1) gene, |
| GenBank Number |
U26257
|
| Species |
Saccharomyces cerevisiae Meyen ex E.C. Hansen |
| Depositors |
SI Reed, Department of Molecular Biology, Scripps Research Institute |
| Vector |
Name of vector: YEp13
Intact vector size (kb): 10.667
Type of vector: plasmid
Cloning sites: BamHI PvuII XhoI
Construction: pBR322, S. cerevisiae LEU2, 2 micron ori
Host range: S. cerevisiae; E. coli
Features:
promoter: none
marker(s): tetR, →, 89-1279
replicon: pMB1, 2540-2540
marker(s): ampR, <-, 3298-4158
marker(s): LEU2, →, 5843-6937
replicon: 2 micron, →, 8988-10329
Cross references: DNA Seq. Acc.: U03498
Distribution host: Escherichia coli RR1 (ATCC® 31343™)
Distributed: Frozen
Growth temperature: 37°C |
| Insert |
Type of insert: genomic
Insert size: 5-20 kb
Type of digest: Sau3A partial
Markers: ampR, LEU2
Number of independent recombinants: 8.20x104
Nonrecombinants (%): 50.0
Number of clones for complete coverage: 1.0x104
Titer: 5.9x1010 cfu/mL |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Frozen glycerol stock of Escherichia coli containing the plasmid library |
| Comments |
A Saccharomyces cerevisiae genomic library in plasmid YEp13 |
| Classification |
Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae |
| References |
Nasmyth KA, Reed SI. Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene. Proc. Natl. Acad. Sci. USA 77: 2119-2123, 1980. PubMed: 6246523
Demianova M, et al. Yeast proteins related to the p40/laminin receptor precursor are essential components of the 40 S ribosomal subunit. J. Biol. Chem. 271: 11383-11391, 1996. PubMed: 8626693
|
