| 產品名稱 |
gt10.1 |
| 商品貨號 |
B203745 |
| Designations |
gt10.1 |
| GenBank Number |
U25652
|
| Species |
Mus musculus, mouse |
| Depositors |
BR Olsen, R Haronen |
| Applications |
contains sequence mouse type XII collgen, alpha1-(XIIB) |
| Vector |
Construct size (kb): 4.586999893188477 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.700000047683716 Tissue: house mouse embryo skin, fibroblast collagen XII Gene product: mouse type XII collgen, alpha1-(XIIB) [Col12a1-XIIB] Target Gene: mouse type XII collgen, alpha1-(XIIB) |
| Insert Size (kb) |
1.700 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.6; EcoRI/HindIII--3.1, 1.5; PstI--4.6; EcoRI--3.1, 1.5. The alpha 1(XII) collagen gene gives rise to two molecular variants, differing in the length of the finger-like regions, by alternative splicing of the primary transcript. The long form is the predominant form at early stages; the short form later stages. The long form is ATCC#63447 and the short form is ATCC#63446. This was constructed by TA cloning of the PCR product. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Bohme K, et al. Primary structure of the long and short splice variants of mouse collagen XII and their tissue-specific expression during embryonic development. Dev. Dyn. 204: 432-445, 1995. PubMed: 8601036
|
